Enzo Life Sciences offers hundreds of immunoassay and enzyme activity assay kits in a variety of formats to service the biomarker and drug discovery assay markets. Our assay offering includes both competitive immunoassays for peptides and small molecules such as cyclic nucleotides and eicosanoids, and immunometric “sandwich” ELISA format assays for large protein analytes including cytokines, cell stress proteins, and signaling pathway regulators. As scientists and manufacturers of kits, we understand the critical nature of your research. Each of our assay kits undergoes rigorous testing to ensure high precision and accuracy while delivering the sensitivity and specificity to detect biologically relevant levels of analyte. Our industry-proven manufacturing capabilities guarantee you will obtain reproducible results, day-after-day and lot-after-lot.
Our decades of experience in the design and manufacture of active enzymes and their substrates supports development of an ever-expanding portfolio of biochemical assays. Our menu of scalable enzyme activity assays includes kits for epigenetic modulation (HDAC, HAT, Sirtuin), matrix metalloproteinases (MMPs) and caspase activity, as well as phosphospecific antibody-based kinase assays. These assays exemplify the synergy in our capabilities, assembling small peptide, active recombinant protein synthesis, fluorescent labeling and detection technologies, and antibody development into robust, reproducible assay kits.
Detect Small And Large Analytes Accurately & Efficiently
Competitive & immunometric formats
Multi-plate packs available
Color coded reagents reduce user error
Amp’d™ Technology to improve ELISA sensitivity
Our catalog of nearly 300 ELISA kits includes sensitive, specific assays for relevant markers of cell viability, signaling pathways, steroid and peptide hormones, inflammation, and more. In addition to traditional antibody pair based immunometric assays, our scientists specialize in the particularly challenging production of high-specificity competitive ELISAs for detection of small molecules. Over 20 years of assay development experience and state-of-the-art manufacturing facilities ensure time-tested reproducibility.
Competetive ELISA Format for Small Molecules & Peptides
Immunometric ELISA Format for Large Molecules
Visualize Cellular Responses
Novel assays for autophagy, aggregation, and multi-drug resistance
Increased photo-stability and permeability
Multiplex compatible (GFP-Certified?) assays
Our CELLESTIAL? portfolio of fluorescent probes and assay kits for cellular analysis provides a complete set of tools for monitoring cell viability, proliferation, death, oxidative stress and toxicology. Our dye-based assays have been optimized for the most demanding imaging applications including confocal microscopy, wide-field fluorescence microscopy, flow cytometry, and high content screening, where consistency and reproducibility are essential.
Autophagy detected in living cells
GFP compabtible Apoptosis detection
Enzyme Activity Assays
Screen Enzyme Modulators With Innovative Activity Assays
High purity, high activity control enzymes
Novel substrate/developer chemistry
Reduce interference from candidate compounds
Whether you are performing large-scale screens, or studying specific enzyme kinetics, our high activity enzymes, unique substrates, and convenient kits are designed to deliver high-quality hits.
Modified Substrate-Based Format
Rapid Generation of Monoclonal Antibodies
A Novel Technology for mAb Detection and Isolation
The Direct Selection of Hybridomas (DiSH) kit is a novel system in which mAb-secreting hybridoma cells co-express significant amounts of the membrane form of the secreted immunoglobulin (Ig) on their surfaces. Subsequently, hybridomas with high-affinity for the target antigen are labeled. And once labeled, these hybridomas can be isolated from among a pool of hundreds of thousands of cells in a matter of hours using Fluorescence activated cell sorting (FACS) or magnetic separation. The total elapsed time from harvest to the first screening is typically 16 days, resulting in improved efficiency for detection of highly specific mAb.
Key Features of the Technology:
Decreased time and labor costs
Improved selection of specific, stable hybridomas
Minimal manipulation of cloned cell lines relative to approaches requiring Limiting Dilution Subcloning